Biomarker Platform
The group IIMD-23 “Biomedical Research Group on Respiratory Infection and Sepsis (BioSepsis)” at IBSAL offers a specialized service for the precise quantification of nucleic acids and proteins in biological samples using advanced digital PCR, SimplePlex® immunoassay, and ELISA techniques.
Description
The identification of biomarkers and molecular profiles is essential for advancing our understanding of the biological mechanisms underlying various diseases, as well as for the development of innovative diagnostic, prognostic, and therapeutic strategies.
This service is available to researchers in any scientific field—whether basic, clinical, or translational—who are affiliated with IBSAL or external institutions.
Portfolio of services
- Extraction and Quantification of Nucleic Acids (RNA/DNA)
- Absolute quantification of nucleic acids
- Detection of Multiplexed Protein Profiles
- Determination of Protein Biomarkers Using ELISA
- Optimization and Validation of Protocols for ddPCR, SimplePlex®, and ELISA
- Scientific and technical advice and experimental design:
- Analysis and Interpretation of the Results
Available Equipment
- Digital PCR: QX200 Droplet Digital PCR System (Bio-Rad)
- Digital PCR: C1000 Touch Cycler with 96-well DP RM
- Digital PCR: PX1 PCR Plate Sealer
- Digital PCR: QX Manager Standard Edition Software
- Nucleic Acid Quantification: Invitrogen™ Qubit™ 4 Fluorometer (Thermo Fisher Scientific)
- SimplePlex® Immunoassay: Ella Platform (ProteinSimple, Bio-Techne)
- SimplePlex® Immunoassay: SimplePlex Runner Software 3.7.2.0
- SimplePlex® Immunoassay: SimplePlex Explorer Software 3.7.2.0
- ELISA Immunoassay: MB-580 Microplate Reader (Heales)
- Two TELSTAR-BIO II Advance Plus biosafety cabinets for sample handling
- 37 °C Incubator, LLG-uniINCU 28 cool
- 5425 R Refrigerated Centrifuge
- -80°C Freezers: EDWAL 405-570L (Thermo) and CryoCube F570n, 570 L (Eppendorf)
- Liebherr Combi Refrigerator (4°C and -20°C) and Siemens Refrigerator (4°C) for storing reagents and samples
- Micropipettes and other laboratory equipment needed for sample processing and performing the techniques
